Antimicrobial Effect of Combined Extract of Three Plants Camellia Sinensis, Teucrium Polium and Piper Nigrum on Antibiotic Resistant Pathogenic Bacteria

Background and Aims: Microbial biofilms are responsible for 65% of human infections and antibiotic resistance. Therefore, finding appropriate ways to prevent infection and biofilm formation is essential. Medicinal plants are one of the suitable candidates to inhibit the antibiotics resistance particularly in biofilm forms. In this study, antimicrobial effects of T.C.P combined extracts (methanolic and ethanolic) were evaluated for 6 antibiotic resistant bacteria in planktonic and biofilm forms. Materials and Methods: The antibacterial activity of extracts on planktonic and biofilm form of antibiotic resistant bacteria, were evaluated by disk diffusion method, macrobroth dilution technique and microtiter plate method. Results: According to disc diffusion test (MBC and MIC), extracts were efficient for inhibition of planktonic forms of bacteria. Although the ethanolic extract was more effective compared to the methanol extract. The T.C.P combined extracts could inhibit biofilm formation and destruct synthetized biofilms. Inhibitory effects on metabolic activity of bacteria had a direct association with the concentration of extract. The maximum inhibitory effects of T.C.P combined extracts on biofilm formation, destruction of synthetized biofilms and inhibition of metabolic activity were observed for S. aureus, (98.13%), S. aureus, (96.3%) and E. coli (81.16%) respectively.  Conclusion: T.C.P combined extracts can be used as an alternative component with inhibitory antibiotic resistant bacteria in planktonic and biofilm form.